36 research outputs found

    Association between GNRHR, LHR and IGF1 polymorphisms and timing of puberty in male Angus cattle

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    <p>Abstract</p> <p>Background</p> <p>In bovines, there are significant differences within and among beef breeds in the time when bulls reach puberty. Although the timing of puberty is likely to be a multigenic trait, previous studies indicate that there may also be single genes that exert major effects on the timing of puberty within the general population. Despite its economic importance, there are not many SNPs or genetic markers associated with the age of puberty in male cattle. In the present work, we selected three candidate genes, <it>GNRHR</it>, <it>LHR </it>and <it>IGF1</it>, and associated their polymorphisms with the age of puberty in Angus male cattle.</p> <p>Results</p> <p>After weaning, 276 Angus males were measured every month for weight (W), scrotal circumference (SC), sperm concentration (C) and percentage of motility (M). A total of 4 SNPs, two within <it>GNRHR</it>, one in <it>LHR </it>and one in <it>IGF1 </it>were genotyped using the pyrosequencing technique. <it>IGF1-SnaBI SNP </it>was significant associated (P < 0.01) with age at SC 28 cm, but it were not associated with age at M 10% and C 50 million. Genotype <it>CC </it>exhibited an average age at SC 28 cm of 7 and 11 days higher than <it>CT </it>(p = 0.037) and <it>TT </it>(p = 0.012), respectively. This SNP explained 1.5% of the genetic variance of age of puberty at SC28. <it>LHR-I499L</it>, <it>GNRHR-SNP5 </it>and <it>GNRHR-SNP6 </it>were not associated with any of the measurements. However, <it>GNRHR </it>haplotypes showed a suggestive association with age at SC 28 cm.</p> <p>Conclusions</p> <p>The findings presented here could support the hypothesis that <it>IGF1 </it>is a regulator of the arrival to puberty in male calves and is involved in the events that precede and initiate puberty in bull calves. Given that most studies in cattle, as well as in other mammals, were done in female, the present results are the first evidence of markers associated with age at puberty in male cattle.</p

    Refining genomewide association for growth and fat deposition traits in an F₂ pig population

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    The identification of genomic regions that affect additive genetic variation and contain genes involved in controlling growth and fat deposition has enormous impact in the farm animal industry (e.g., carcass merit and meat quality). Therefore, a genomewide association study was implemented in an F₂ pig population using a 60,000 SNP marker panel for traits related to growth and fat deposition. Estimated genomic EBV were linearly transformed to calculate SNP effects and to identify genomic positions possibly associated with the genetic variability of each trait. Genomic segments were then defined considering the markers included in a region 1 Mb up- and downstream from the SNP with the smallest -value and a false discovery rate < 0.05 for each trait. The significance for each 2-Mb segment was tested using the Bonferroni correction. Significant SNP were detected on SSC2, SSC3, SSC5, and SSC6, but 2-Mb segment significant effects were observed on SSC3 for weight at birth (wt_birth) and on SSC6 for 10th-rib backfat and last-rib backfat measured by ultrasound at different ages. Furthermore, a 6-Mb segment on SSC6 was also considered because the 2-Mb segments for 10 different fat deposition traits were overlapped. Although the segment effects for each trait remain significant, the proportion of additive variance explained by this larger segment was slightly smaller in some traits. In general, the results confirm the presence of genetic variability for wt_birth on SSC3 (18.0-20.2 Mb) and for fat deposition traits on SSC6 (133.8-136.0 Mb). Within these regions, fibrosin (FBRS) and myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF) genes could be considered as candidates for the wt_birth signal on SSC3, and the SERPINE1 mRNAbinding protein 1 gene (SERBP1) may be a candidate for the fat deposition trait signals on SSC6.Facultad de Ciencias VeterinariasInstituto de Genética Veterinari
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